Fig. 6

SPR analysis of bNAb binding to bNAb-purified and -depleted BG505 and B41 SOSIP.664. Sensorgrams for individual bNAbs are shown. A BG505 (top) and B41 (bottom) SOSIP.664 trimers were affinity-purified the bNAbs 2G12, PGT151, or PGT145 in a first step and thereafter by SEC. To the right in the B41 row is the sensorgam for the autologously neutralizing rabbit mNAb 16D. Each sensorgram shows the response after background subtraction on the y axis (response units, RU) over time after start of injection on the axis (s). Association was monitored for 300 s and dissociation for 600 s. B B41 SOSIP trimer was first 2G12-affinity and then SEC-purified and thereafter depleted by passage through Sepharose columns with the bNAbs PGT151 or PGT145 or no antibody on the beads. The flowthrough trimer was immobilized and analyzed by SPR as in A. The sensorgrams represent 2–3 highly similar replicates