Fig. 5

B41 SOSIP.664 trimer. A A surface-rendered model of the B41 SOSIP.664 trimer (PDB 6MCO [52]) oriented with the apex up (top model) or viewed from above (bottom model). The peptidic surface is gray on one protomer and light blue on the other two. Man₉ glycans were added to the published model [52] and are depicted as sticks and colored green unless they are directly involved in the epitopes of the three bNAbs used for affinity purification, in which case they are colored as the rest of the epitope. Contacts with PGT145 are colored blue, with 2G12 yellow, and with PGT151 purple. N289, which is not part of a PNGS in B41 SOSIP.664, is colored magenta. B Purified Env proteins were analyzed by electrophoresis in 4–12% Bis–Tris BN-PAGE gels with Coomassie-blue staining. 2 μg protein per well was loaded from each purification. C NS-EM analyses of unliganded the B41 SOSIP.664 trimer purified by PGT151-affinity chromatography. The propeller-like, triangular particles show 100% native-like trimer molecules