Attenuation of reverse transcriptase facilitates SAMHD1 restriction of HIV-1 in cycling cells

Retrovirology

Table 1 SAMHD1 catalytic turnover and tetramer stability of T592 mutants

SAMHD1	Substrate	AL1	AL2	k_cat (s⁻¹)	f_tet^b (3 h)	(k_dec)^c(s⁻¹) × 10⁵	(k_cat/k_dec)
w.t	dATP	GTP	dATP	0.864 ± 0.092^a	0.38	7.02	12,308
T592A	dATP	GTP	dATP	0.087 ± 0.029	0.50	5.14	1692
T592C	dATP	GTP	dATP	0.096 ± 0.021	0.63	2.94	3265
T592D	dATP	GTP	dATP	0.100 ± 0.012	0.19	11.2	*893*
T592E	dATP	GTP	dATP	0.111 ± 0.018	0.36	7.00	1586
T592I	dATP	GTP	dATP	0.101 ± 0.009	0.63	3.07	3289
T592K	dATP	GTP	dATP	0.120 ± 0.019	0.12	22.1	*543*
T592L	dATP	GTP	dATP	0.127 ± 0.042	0.20	15.2	*836*
T592S	dATP	GTP	dATP	0.023 ± 0.004	0.68	2.33	*987*
T592V	dATP	GTP	dATP	0.104 ± 0.025	0.61	2.53	4111

^aError is the SEM of at least three independent measurements
^bFraction tetramer remaining after 3 h incubation
^cTetramer decay rate determined from fitting the f_tet between 5 min and 3 h to an exponential decay function Y = Ae^−kdec.x. k_cat/k_dec values are grouped as WT to WT/4 (plain text), WT/6 to WT/8 (Italics) and < WT/12 (Bold-italics)

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