Fig. 2
From: Attenuation of reverse transcriptase facilitates SAMHD1 restriction of HIV-1 in cycling cells
Tetramer stability of T592 mutants SEC–MALLS analysis of tetramer stability for (A) WT SAMHD1 and (B–J) SAMHD1 T592 mutants after addition of GTP and dATP nucleotides. The solid lines are the chromatograms from the output of the differential refractometer and the black scatter points are the weight-averaged molar masses determined at 1 s intervals throughout elution of chromatographic peaks, SAMHD1 monomer-dimers elute at 13.0–14.0 min, tetramers at 10.5–11.0 min. The displayed chromatograms in each panel are (red) apo-SAMHD; (black) SAMHD1 and 0.2 mM GTP, 0.5 mM dATP after 5 min incubation; (blue) SAMHD1 and 0.2 mM GTP, 0.5 mM dATP after 60 min incubation; (cyan) SAMHD1 and 0.2 mM GTP, 0.5 mM dATP after 180 min incubation. The side chain configuration of the amino acid substitutions at T592 are displayed adjacent to the chromatograms