Fig. 1

Time dependence of Vpx rescue of HIV-1 infection (A) Time course of Vpx rescue of HIV-GFP infection. Vpx was introduced into either differentiated parental U937 cells (red line) or cells expressing WT SAMHD1 (Blue line) or the inactive SAMHD1(1–583) mutant (green line), co-incident with HIV-GFP or at intervals up to 48 h post-infection. Cells were harvested at 96 h post-infection and analysed for GFP expression by flow cytometry. The plot shows the ratio of Vpx⁺ cells expressing GFP relative to Vpx⁻ cells expressing GFP, error bars represent the range of at least three independent experiments. A ratio of one indicates that the presence of Vpx had no effect on HIV-GFP infection. B Nevirapine washout assay. Differentiated U937 cells were treated with media (red line) or media containing 10 µM Nevirapine (blue line) before infection with HIV-GFP. At the indicated time post-infection, the media was changed to remove the drug. Cells were harvested at 96 h post-infection and analysed for GFP expression using flow cytometry. The percentage of GFP⁺ cells are plotted, error bars represent the range of at least three independent experiments