Fig. 3

F-MuLV- and SFFV-infected cells in infected BALB/c mice. Highly susceptible BALB/c mice were infected with 500 SFFU of FV-Katushka-mTagBFP, and infected erythroblasts (Ery), B cells, Gr1⁻ (Gr1⁻ My) and Gr1⁺ myeloid cells (Gr1⁺ My), dendritic cells (DC), CD4⁺ T cells and CD8⁺ T cells in the bone marrow (A), spleen (B) and lymph nodes (C) were analyzed by flow cytometry on days 4, 7, 10, 14 and 21. The frequencies of F-MuLV-Katushka single-infected, SFFV-mTagBFP single-infected and F-MuLV-Katushka, SFFV-mTagBFP double-infected as well as uninfected cells are shown as indicated. Each dot indicates an individual mouse, bars indicate median values, the dotted line indicates the detection limit. Data from 6 (days 4, 7, 10, 21) or 9 (day 14) mice per group were obtained in 1–2 independent experiments per time point. Asterisk indicates statistically significant differences between the indicated types of single or double-infected cells (p < 0.05, multiple t-test with Bonferroni correction for multiple comparisons). Number sign indicates dominant subsets of single or double-infected cells in the respective organ, i.e. infected cell types that were significantly higher than at least three other cell types of the same single or double-infected state (p < 0.05, Kruskal–Wallis ANOVA on Ranks, Dunn’s post test). Dagger indicates statistically significant differences between the respective cell subsets in the bone marrow and spleen, double dagger indicates statistically significant differences between the respective cell subsets in the bone marrow and lymph nodes, inverted cross symbol indicates statistically significant differences between the respective cell subsets in the spleen and lymph nodes (p < 0.05, multiple t-test with Bonferroni correction for multiple comparisons)