Fig. 8
The levels of BST-2 in cells in the presence of β-coronavirus E proteins. HEK293 cells were co-transfected with either the empty pcDNA3.1( +) vector, the pcDNA3.1( +) vector expressing each of the four E proteins, or HIV-1 Vpu and a vector expressing human BST-2 protein. At 30 h post-transfection, cells were starved for methionine/cysteine and radiolabeled with 500 μCi of 35S-methionine/cysteine for 16 h. Cell lysates were prepared in RIPA buffer. One-third of the lysate was used to immunoprecipitate BST-2 proteins using an anti-BST-2 antibody (A); one-third of the lysate was used to immunoprecipitate E proteins and Vpu (B), and one-third of the lysate was used to immunoprecipitate GAPDH to normalize loading (C). The line in Panel A is due to the removal of several lanes from the center of the autoradiograph