Fig. 3

HIV-1 reactivation and TASOR phosphorylation are independent in the J-Lat A1 model of latency. A J-Lat A1 silenced for TASOR expression by CRISPR/Cas9 were sorted by flow cytometry into three populations according to GFP expression, which is a read-out for HIV reactivation (Neg: no GFP expression; DIM: low GFP expression, Bright: high GFP expression). After amplification of the three cell populations for 2 weeks, cells were re-analyzed by flow cytometry and western blot. The higher is the percentage of GFP-positive cells, the loser TASOR is expressed. B J-Lat A1 cells prepared as explained in A were left untreated (DMSO) or treated with nocodazole or etoposide. Cells were harvested to analyze TASOR phosphorylation by western blot (B) or to determine GFP expression by flow cytometry (C and D). C Effect of nocodazole on HIV-1 reactivation on the different cell populations presented in A (n = 4, the mean of 4 experiments is presented. A two-sided unpaired t-test was used. (ns nonsignificant). D Effect of etoposide on HIV-1 reactivation on the different cell populations presented in A (n = 4, the mean of 4 experiments is presented. A two-sided unpaired t-test was used (ns nonsignificant)