Fig. 5

Histone profile of viral DNA in lymphoid K562 cells resembles that of transcriptionally inactive genes. K562 cells and HeLa cells were infected with IN-D64A or IN-wt reporter virus. Cells were harvested for ChIP 24 h after virus application. Antibodies against specific histones or histone modifications were used. Rabbit IgG was used as an isotype control. ChIP samples were analyzed by qPCR using 2-LTR-specific primers (A), GAPDH-specific primers (B) or beta globin-specific primers (C). Data are shown relative to input DNA. Error bars indicate mean ± SD. Each dot represents an independent replicate