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Fig. 9 | Retrovirology

Fig. 9

From: The key amino acid sites 199–205, 269, 319, 321 and 324 of ALV-K env contribute to the weaker replication capacity of ALV-K than ALV-A

Fig. 9

Recovery test of ALV-K gp85 protein point mutants. a, b Results of the co-IP test and grey value analysis of the interactions between soluble recombinant chimeric gp85 vr3 proteins and the Tva receptor. c, d Results of the co-IP test and grey value analysis of the interactions between restored soluble recombinant chimeric gp85 protein point mutants and the Tva receptor; e the percentages of GFP-positive cells for point mutation recovery mutants were analysed by flow cytometry. Three independent experiments were performed, and the data are shown as the mean ± SD of triplicate samples from a representative experiment. Statistical analysis (two-way analysis of variance) was performed using GraphPad Prism 7; *P < 0.05, **P < 0.01

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Retrovirology

ISSN: 1742-4690

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