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Fig. 6 | Retrovirology

Fig. 6

From: The key amino acid sites 199–205, 269, 319, 321 and 324 of ALV-K env contribute to the weaker replication capacity of ALV-K than ALV-A

Fig. 6

Analysis of the binding of recombinant gp85 s8 protein point mutants for the Tva receptor. a, b Co-IP test results and grey value analysis results for the interactions between soluble recombinant chimeric gp85 proteins and the Tva receptor; c evaluation of the efficiency of soluble recombinant chimeric gp85 proteins in competitively blocking RCASBP(A)-EGFP infection. Three independent experiments were performed, and the data are shown as the mean ± SD of triplicate samples from a representative experiment. Statistical analysis (two-way analysis of variance) was performed using GraphPad Prism 7; *P < 0.05, ****P < 0.0001, ns P > 0.05

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