Fig. 3

The subcellular distribution of HIV-1 RNAs is not affected by YTHDC1 knockdown. A HeLa/LAV cells were transfected with a control (Ctrl) or YTHDC1-targeting siRNA (DC1-1). 3 days later, cells were fractionated and the purity of the fractions was assessed by western blot using antibodies against the nuclear protein LEDGF/p75 and the cytoplasmic protein GAPDH. Low level of nuclear contamination by cytoplasmic GAPDH can be observed. YTHDC1 depletion was also monitored using an anti-YTHDC1 antibody. B The relative abundance of US, IS and MS HIV-1 RNAs in the nucleus and in the cytoplasm was monitored by RT-qPCR, and the nuclear to cytoplasmic ratio of HIV-1 RNAs was represented. Data are presented as mean ± S.D. (n = 4). P values calculated using paired t-test were not significant