Fig. 6

Inhibition of NF-κB is conserved amongst Vpx species variants. A NF-κB reporter activity and immunoblot from HEK293T cells co-transfected for 24 h with 50 ng Vpx proteins from SIVmac strain 251, SIVsm strain E543 or HIV-2 or EV control and 50 ng p65 per well. Vpx expression was detected using an anti-Vpx antibody. B NF-κB reporter activity and immunoblot from HEK293T cells co-transfected for 24 h with 50 ng FLAG-tagged Vpx proteins from SIVmac strain 251, SIVrcm or SIVmnd-2 or EV control and 50 ng p65 per well. Vpx expression was detected using an anti-FLAG antibody. C NF-κB reporter activity and immunoblot from HEK293T cells co-transfected for 24 h with 50 ng FLAG-tagged Vpx protein from SIVmac strain 251 or FLAG-tagged Vpr proteins from SIVagm_VER or SIVmon or EV control and 50 ng p65 per well. Vpx/Vpr expression was detected using an anti-FLAG antibody. D NF-κB reporter activity from HEK293T cells co-transfected for 24 h with 50 ng FLAG-tagged Vpx or Vpr proteins in the absence of stimulation. Data are mean ± SD, n = 3, representative of at least 3 repeats. Statistical analyses were performed using Student’s t‐test, with Welch’s correction where appropriate. *P < 0.05, **P < 0.01, ***P < 0.001