Fig. 2

Vpx inhibits NF-κB at the level, or downstream, of p65. A Schematic of NF-κB activation downstream of the TNF receptor (TNFR), IL-1 receptor (IL-1R) and Toll-like receptors (TLRs). B NF-κB reporter activity and representative immunoblot from HEK293T cells co-transfected for 24 h with 25–100 ng SIVmac Vpx or EV control (100 ng) and 25 ng of TRAF2. Vpx and TRAF2 expression was detected with an anti-FLAG antibody. C NF-κB reporter activity and representative immunoblot from HEK293T cells co-transfected for 24 h with 25–100 ng SIVmac Vpx or EV control (100 ng) and 25 ng of TRAF6. Vpx and TRAF6 expression was detected with an anti-FLAG antibody. D NF-κB reporter activity and representative immunoblot from HEK293T cells co-transfected for 24 h with 25–100 ng SIVmac Vpx or EV control (100 ng) and 50 ng of IKKβ. IKKβ and Vpx expression were detected with anti-HA and anti-FLAG antibodies respectively. E NF-κB reporter activity and representative immunoblot from HEK293T cells co-transfected for 24 h with 25–100 ng SIVmac Vpx or EV control (100 ng) and 25 ng of p65. p65 and Vpx expression were detected with anti-HA and anti-FLAG antibodies respectively. F NF-κB reporter activity and representative immunoblot from HEK293T cells co-transfected for 24 h with 25–100 ng SIVmac Vpx or EV control (100 ng) in the absence of stimulation. Vpx expression was detected with an anti-FLAG antibody.Data are mean ± SD, n = 3, representative of at least 4 repeats. Statistical analyses were performed using Student’s t‐test, with Welch's correction where appropriate. *P < 0.05, **P < 0.01, ***P < 0.001