Fig. 7
From: A novel Betaretrovirus discovered in cattle with neurological disease and encephalitis
Detection of bovine retrovirus CH15 (BoRV CH15) RNA in cells morphologically compatible with neurons. Chromogenic in situ hybridization of formalin-fixed, paraffin-embedded tissue slides using the BoRV CH15 RNAscope probe. BoRV CH15 RNA (red granular staining) is visible in the cytoplasm of cells and cellular extensions morphologically compatible with neurons. The neurons appear to be morphologically normal, with the maintenance of Nissl substance, and inflammatory cells are absent from the immediate surroundings of positive neurons. A Case 1, medulla oblongata; B case 6, hypothalamus; C case 6, cerebral cortex; D case 7, hippocampus. Neurons with clear signs of necrosis (chromatolytic and eosinophilic cytoplasm, eccentric nuclei, loss of Nissl substance) on hematoxylin and eosin staining (insets) remained negative for BoRV CH15 RNA. E Case 6, medulla oblongata; F case 7, hippocampus