Table 3 Overview of past and current investigations by CRISPR/Cas9 in HIV therapy
From: Editing out HIV: application of gene editing technology to achieve functional cure
Years | Target region | Cell type/organism | References |
|---|---|---|---|
2014 | HIV-1 LTR promoter U3 region | Myeloid lineage cells | Hu et al. [61] |
2015 | CCR5 locus | Primary human T cells | Sather et al. [52] |
2016 | Heteroduplex of wild type and mutant CCR5 delta 32 (i) Middle band (ii) Upper band (iii) Lower band | (i) Human embryonic kidney HEK 293T cells (ii) Human acute T cell (iii) Leukemia cell line (iv) Human breast adenocarcinoma cell line MDA-MB-231 cells | Qi et al. [111] |
2017 | Gene KO in both mouse and human T cells | CD4+ and CD8+ T cells from mouse and human | Seki et al. [112] |
2017 | GPI-scFv X5 | CD4 cells in hu-PBL mice sand mice with GPI-scFv AB65-transduction | Ye et al. [113] |
2017 | Human CCR5 locus in peripheral lymphocytes from long-term reconstituted mice | Human CD34+ cells | Xu et al. [66] |
2017 | Four different sites of the HIV-1 long terminal repeat (LTR) | HEK293T cells in humanized Bone marrow/liver/thymus (BLT) mice with chronic HIV-1 infection | Yin et al. [65] |
2017 | Gene correction and the knock-in of reporter genes into the rat nestin and human DARPP-32 genes | Human embryonic kidney (HEK) 293T and U2OS cells, C6 cells and human adult dermal fibroblasts | Gaj et al. [114] |
2017 | Genetic disruption of Pdcd1 in CAR T cells | Primary human T cells Purified human CD4+ or CD8+ T cells | Rupp et al. [115] |
2017 | Pcsk9 native, 5′ and 3′ and e-sgRNAs targeting mouse fumarylacetoacetate hydrolase (Fah) and ROSA26 loci | HEK293 cells in human | Yin et al. [116] |
2019 | Short single-stranded DNA HDR donor | Mammalian cells: CD34+ HSPCs | Wu et al. [117] |