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Table 3 Thermodynamic properties of PT binding to WT and mutant gp120 variants (HxBc2 strain)

From: Altered Env conformational dynamics as a mechanism of resistance to peptide-triazole HIV-1 inactivators

 

ITCa

KD (nM)

ΔH (kcal/mol)

ΔG (kcal/mol)

−TΔS (kcal/mol)

n

AAR029b

 WT

25 ± 2.0

− 14 ± 0.37

− 10 ± 0.52

4.5 ± 0.90

1.1 ± 0.04

 V255I

220 ± 2.0

− 9.5 ± 0.29

− 9.1 ± 0.33

0.43 ± 0.30

1.0 ± 0.01

 V255I/S143N

230 ± 12

− 10 ± 0.49

− 9.1 ± 0.42

1.0 ± 0.91

1.1 ± 0.04

KR13

 WT

16 ± 1.4

− 19 ± 1.7

− 11 ± 4.7

7.8 ± 3.0

1.1 ± 0.01

 V255I

59 ± 9.2

− 13 ± 0.86

− 10 ± 0.61

2.9 ± 1.5

1.0 ± 0.04

 V255I/S143N

59 ± 0.60

− 12 ± 0.62

− 10 ± 0.31

1.9 ± 0.93

1.1 ± 0.08

  1. aITC data: mean of two replicates ± range of mean
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Retrovirology

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