Fig. 1
From: An emerging and variant viral promoter of HIV-1 subtype C exhibits low-level gene expression noise
RBEIII duplication reduces background noise. Schematic representation of a panel of Tat- and Rev-dependent dual-reporter expression (mScarlet and G-Luc) lentiviral vectors. mScarlet (d2mScarlet) contains a degradation domain. The reporter gene expression cassette and the RRE motif are flanked by the splice donor and acceptor elements. The NF-κB motifs are highlighted by green background color and the RBEIII sites by red color. Dashes in the multi-sequence alignment indicate sequence deletion and dots sequence identity. N and R represent the NF-κB and RBF-2 transcription factor binding sites, respectively