Fig. 2

Reactivity of the IgG1 and IgG3 forms of 1E5 to global panel of HIV-1 Envs. HEK 293T cells were transiently transfected with plasmids expressing genes for both Env and EGFP. At 48 h post-transfection, cells were stained with the test IgG 1E5 (IgG1 and IgG3 forms) and three control IgGs: A32 (cluster A), VRC01 (CD4bs) and 4E9C (CoRBS). Cells were then fluorescently labeled with an APC-conjugated anti-human IgG secondary Ab. Histograms of APC in EGFP⁺ cells stained with test IgG and NHG as a negative control are shown by a red line and gray shading, respectively