A novel class III endogenous retrovirus with a class I envelope gene in African frogs with an intact genome and developmentally regulated transcripts in Xenopus tropicalis

Table 1 Primers used for cloning the XtERV-S provirus and for expression studies

Primer	Sequence (5′–3′)
Cloning XtERV-S
XP1 F	CTC GAG GAT TCC CCA AGT CAC ATG AGA TG
XP1 R	GCG GCC GCG ACT TAC TGC TGG CCG AAA TAC TC
XP2 F	CTC GAG CTG TCA ATC TCA AGC AGT ATC GCA TAC
XP2 R	GCG GCC GCC CAC TCA GGG TCG GTT CTG TTT ATC CAA CTC
XP3 F	CTC GAG TAA TGT AAC AAA CTG TTG GAT ATG TG
XP3 R	GCG GCC GCT GTA ATA AAG GGG TTA ACC TTT ATC
LTR cloning into pGL3 basic
XtERV-S LTR FW	GGT ACC TGA TTT GTA TGA TTT ACA ATT TAT ACA TG
XtERV-S LTR RE	GGA TCC GTA ATA AAG GGG TTA ACC TTT ATC
MoMLV LTR FW	GGA TCC TGA AAG ACC CCA CCT GTA GGT TTG GCA
MoMLV LTR RE	AAG CTT TGA AAG ACC CCC GCT GAC GGG TAG TC
Gag cloning into eGFPC1
Gag FW	GGA TCC TTA TGT TCT GCT GGT TAA AAA ACA AGG TTA G
Gag Rev	CTC GAG ATC TAT AGG ACT GGG CAC CTC

Underlined regions of the sequence indicate the restriction sites used for cloning

ISSN: 1742-4690