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Fig. 7 | Retrovirology

Fig. 7

From: A novel class III endogenous retrovirus with a class I envelope gene in African frogs with an intact genome and developmentally regulated transcripts in Xenopus tropicalis

Fig. 7

XtERV-S is transcriptionally active. A Functional analysis of the XtERV-S LTR cloned into a luciferase reporter vector and transfected into 293T cells. Luciferase expression is compared to the promoter-less vector pGL3 basic and to vectors using the Moloney mouse leukemia virus (MoMLV) LTR and CMV promoter. B Confocal examination of GFP-tagged XtERV-S Gag protein in 293T cells shows accumulation in the cytoplasm at the plasma membrane (red arrows) and localization in nucleus (yellow arrows). C ESTs mapping to XtERV-S are expressed embryonically in X. tropicalis. ESTs map to the gag, pol and env regions. D RNAseq reads map mostly to LTR and the gag-pol ORFs. The chart shows the total number of reads per kilobase per million (RPKM) mapped to the provirus and the reads mapping to LTR, gag, pol, and env. The developmental stages and events are indicated, including MBT (mid-blastula transition); the red arrow represents the transcriptional transition from maternal mRNA to zygote genome transcribed mRNAs; the green arrow represents the beginning of the tadpole stage

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Retrovirology

ISSN: 1742-4690

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