Table 4 Primers and probes used in reverse transcriptase PCR to quantify expression of TRIM5α, CypA and GAPDH
Protein | Primers and probes(Tamra) |
|---|---|
TRIM5α F | 5′-TGCCTCTGACACTGACTAAGAAGATG |
TRIM5α R | 5′-GGGCTAAGGACTCATTCATTGG |
TRIM5α Probe | 5′-(6-Fam)AAGCTTTTCAACAGCCTTTCTATATCATCGTGTGATA |
CypA F | 5′-GGCCGCGTCTCCTTTGA |
CypA R | 5′-AATCCTTTCTCTCCAGTGCTCAGA |
Probe | (6-Fam)TGCAGACAAGGTCCCAAAGACAGCAG |
GAPDH F | 5′-ACCCCTGGCCAAGGTCATC |
GAPDH R | 5′-AGGGGCCATCCACAGTCTTC |
Probe | 5′-(6-Fam)AGGACTCATGACCACAGTCCATGCCA |