Fig. 3

Infectivity of HIV-1 Gag-NanoLuc particles can be rescued by co-expression with WT Gag. a RT activity of virus particles produced in HEK293T cells transfected with either WT pNL4-3, pNL4-3 Gag-iGFP, or the indicated pNL4-3 Gag-NanoLuc vectors. RT activity is shown relative to the WT HIV-1. b Infectivity of virus particles from (A) in the TZM-bl indicator cells at 48 h post-infection. Viral inputs were normalized for RT activity. Infectivity is shown relative to WT HIV-1. c Infectivity in TZM-bl cells of virus produced in HEK293T cells transfected with either WT pNL4-3, pNL4-3 Gag-iNanoLuc, or both pNL4-3 and pNL4-3 Gag-iNanoLuc at indicated ratios of pNL4-3 to pNL4-3 Gag-iNanoLuc, with amount of total input DNA held constant. Viral inputs were normalized for RT activity. Infectivity is shown relative to WT HIV-1(pNL4-3). *, P < 0.05 by one-way ANOVA compared to WT pNL4-3 infectivity. d FLuc and NanoLuc activity from lysates of infected TZM-bl cells from c. Error bars ± SD, n = 3 independent experiments for all panels