Fig. 2From: Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latencySingle-cell multiplex qPCR analysis of HIV cell line models. a Principal component analysis of the gene expression levels (right plot: dimension 2 vs. dimension 1; left: dimension 3 vs. dimension 1). Single cells for each cell line are indicated by color and different shapes denote independent assays (batch). No batch effects were observed (P < 0.001, Wilcoxon rank sum test). b Scatter plots of average expression levels (40-cycle threshold; ~ log2) of all targets in single cells (y axis) vs. bulk cells (x axis) for each cell line and overall (box). High concordance was observed (R > 0.86 for all cell lines)Back to article page