Fig. 5

NNRTI-induced killing can be combined with LRA treatment to purge the latent reservoir. a IL-4 treated resting T cells were infected with a GFP reporter virus and cultured for 15 days. IL-4 was replenished on 7 dpi. Fifteen days after infection, GFP- cells were sorted and treated or not with combination of latency reversing agents (prostratin 330 nM and scriptaid 200 nM) plus RPV (1 μM), RPV + NVP (1 μM) or IDV (1 μM). Twenty-four hours later GFP and p24Gag expression were analyzed. Horizontal lines represent the mean of 6 donors from 2 independent experiments (*p = 0.0004; **p = 0.0182; ***p = 0.0051: p-values were calculated with an unpaired two-tailed t-test). b At 5 dpi, total infected cells were treated with a combination of LRA as in A. Twenty-four hours later dead cells were removed by Ficoll, DNA was extracted, and levels of provirus integration was measured by nested Alu-PCR. Data are averages from 3 donors. (*p = 0.0044; **p = 0.0179; ***p = 0.0418; ****p < 0.0001: p-values were calculated with an unpaired two-tailed t-test)