Fig. 3

FUS silences gene transcription from the HIV promoter. a FUS expression in Jurkat cells. Western Blotting analysis confirming endogenous expression of FUS in J-LTR-Luc cells, (lane 1) and expression of Flag-FUS in J-LTR-Luc-FUS cells (lane 2) using FUS IgG. b FUS silences transcription from the HIV promoter. Jurkat (J)-LTR-Luc and J-LTR-Tat-Luc cells that stably express Tat, were monitored for their LTR luciferase readings in the absence or presence of FUS expression (gray bars), or its SGG4 mutant that does not bind RNA (black bars). Relative transcription corresponds to luciferase readings relatively to control Jurkat cells that express the LTR-Luc reporter gene - J-LTR-Luc - set to 1 (white bars). Readings are representative of three independent experiments. The error bars represent mean ± SD from three independent reactions. Asterisks indicate levels of statistical significance as calculated by two-tailed student T test (**p ≤ 0.01). c Western Blot analysis confirming Flag-FUS (lanes 2–3) and SGG4 (5–6) expression in J-LTR-Luc and J-LTR-Tat-Luc cells using anti-Flag IgG. Lanes 1 and 3 represent cells that do not express Flag-FUS. Lower panel represent tubulin western blot for monitoring loading control. d Western Blot analysis to confirm equal expression of Tat in J-LTR Tat-Luc and J-LTR-Tat-Luc-FUS (lanes 2 and 3) and compared to J-LTR -Luc that do not express Tat (lane 1). Lower panel represent tubulin western blot for loading control