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Fig. 6 | Retrovirology

Fig. 6

From: CCR5 editing by Staphylococcus aureus Cas9 in human primary CD4+ T cells and hematopoietic stem/progenitor cells promotes HIV-1 resistance and CD4+ T cell enrichment in humanized mice

Fig. 6

CCR5-editing in the human hematopoietic stem cells by LentiCRISPR/SaCas9 maintains potential multilineage ability. a CCR5 gene editing by LentiCRISPR/SaCas9-sgRNA-#8 or control in CD34+ HSPCs analyzed via T7E1 assay. b Sanger sequencing of CCR5 gene target loci in modified primary CD34+ HSPCs. c Numbers of colony types generated from untreated and CCR5-modified CD34+ HSPCs by CFU assay. Data were analyzed by unpaired t-test and error bars showed the mean ± SEM of three independent experiments

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Retrovirology

ISSN: 1742-4690

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