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Fig. 5 | Retrovirology

Fig. 5

From: Interaction of HIV-1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV-1 replication

Fig. 5

Quantitative PCR to analyse the impact of PSF knockdown on HIV-1 2-LTR, integrated provirus and late RT products. TZM-bl cell line was transduced with the pNL4-3 at 1 MOI. a 2-LTR detection at different time point in the PSF (siPSF) knockdown and control cells (siScrambled). Paired t test analysis revealed p values < 0.03. b Integrated provirus was detected by nested Alu PCR in PSF knockdown cells. Paired t test analysis revealed p values < 0.04. Error bar depicts SD between three independent experiment. c Viral cDNA was detected by qPCR at different time points. Error bar shows the SD between three independent experiment (*p < 0.05)

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Retrovirology

ISSN: 1742-4690

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