Fig. 3

Fluorescent images of Env-isfGFP-∆V1V2 in lymphoblastoid cell line, Jurkat E6. Jurkat cell transfected Env-isfGFP-∆V1V2 plus NL4-3 (a–c), or with NL4-3 only (d), or with Env-isfGFP-∆V1V2 plus Gag-iCherry (f–i) were fixed, permeabilized and stained with anti-Env mAb 2G12 (a–d) or Cell Mask Deep Red (f–i). Fluorescence intensity across co-transfected Jurkat cell shown in merged panel of (c) and (i) were displayed in (e) and (j). k and l Live time-lapse confocal fluorescence imaging of an Env-isfGFP-∆V1V2-expressing Jurkat lymphoblastoid T cell. Confocal z stacks were acquired at 10-min intervals from 5 h post transfection for 30 h. k Montage of fluorescence images of one cell (cell 9) illustrating changes in the fluorescence pattern of Env-isfGFP-∆V1V2 at indicated time points marked in (l). l Graph of fluorescence intensity of Env-isfGFP-∆V1V2-expressing cells in one field over time. Mean intensities above background was plotted. The expression of most cells peaked between 20-25 h post transfection. Red X indicates cell death of cell 1 and 2 and O indicates cell 9 moves out of field. Bar = 3 μm