Table 1 Sequence of primers and probes used to detect polymorphisms of the promoter and structural regions of the MBL2 gene
From: MBL2 gene polymorphisms in HHV-8 infection in people living with HIV/AIDS
MBL2 gene region | Nucleotide sequence |
|---|---|
Promoter region (-550) | |
Primer forward | 5′-CCAACGTAGTAAGAAATTTCCAGAGA-3′ |
Primer reverse | 5′-CAACCCAGCCCAGAATTAACTG-3′ |
Sonda VIC | 5′-VIC-CCTGTCTAAAACACC-MGB-3 |
Sonda FAM | 5′-FAM-AGCCTGTGTAAAAC-MGB-3 |
Promoter region (-221) | |
Primer forward | 5′-GCACGGTCCCATTTGTTCTCA-3′ |
Primer reverse | 5′-GCGTTGCTGCTGGAAGACTATAAA-3′ |
Sonda VIC | 5′-VIC-CATGCTTTCGGTGGCAG-MGB-3′ |
Sonda FAM | 5′-FAM-CATGCTTTCCGTGGCAG-MGB-3′ |
Structural region (exon-1) | |
Primer forward | 5′-AGGCATCAACGGCTTCCCA-3′ |
Primer reverse | 5′-CAGAACAGCCCAACACGTACCT-3′ |