Fig. 5

Preferential expression of CXCL10 in Human T-cell leukemia virus type-1 (HTLV-1)-infected T-cell lines derived from patients with adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). a Expression of CXCL10 mRNA was examined by real-time PCR in HTLV-1-infected and -uninfected T-cell lines. CXCL10 mRNA was preferentially expressed in HTLV-1-infected human T-cell lines derived from patients with ATL (3 out of 5 tested) and HAM/TSP (4 out of 4 tested), compared with HTLV-1-transformed T-cell lines (0 out of 3) and HTLV-1 negative human T-cell lines (0 out of 3). b The expressions of tax and HBZ mRNA were examined by quantitative real-time PCR in HTLV-1-infected T-cell lines. The expression levels of the viral RNAs tax and HBZ were relatively high in T-cell lines derived from patients with HAM/TSP and HTLV-1-transformed T-cell lines when compared with those in ATL cell lines. c CXCL10 levels in culture supernatants from HTLV-1-infected and -uninfected human T-cell lines were assessed by ELISA. Significant levels of CXCL10 was observed in the cultured supernatants from HTLV-1-infected human T-cell lines derived from patients with ATL and HAM/TSP, whereas it was not detectable in any of the other cell lines tested. d Expression of Tax and HBZ was examined by Western blot in HTLV-1-infected and -uninfected T-cell lines