Fig. 2
From: RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency
Protective shRNAs inhibit reactivation of latent HIV-1. Parental and transduced J-Lat 9.2 cells were quantified for shRNA expression a and then treated with increasing concentrations of b TNF, c SAHA, d SAHA and TNF, e Bryostatin and TNF, f Chaetocin, g DZNep,. All treatments went for 48 h, except from Chaetocin and DZNep, which were for 72 h. Percentage of GFP + cells was measured through flow cytometry from the live (Parental) or live/mCherry + cells (transduced cell lines) and a total of 10,000 events were collected from each sample from the live cell population. The relevant concentrations of TNF and SAHA are indicated in the corresponding plots. Comparisons shown were performed against the Parental cell line using the Kruskal–Wallis Multiple comparison test, correcting for multiple comparison with Dunn’s test (*p ≤ 0.05)(Mean ± SD). Data shown is from at least one independent representative experiment performed in triplicate