Fig. 2

Kudzu blocks HIV-1 replication of R5 tropic viruses. a Activity of Kudzu on acute replication of GHOST-CCR5 cells with JRCSF and YU2 viruses. Cells were infected with both viruses in presence of 2 potent dilutions of Kudzu. Viral supernatants recovered 72 h post-infection from cells were assayed for their p24 antigen content. Raltegravir (Ralt, an integrase inhibitor, 100 nM) and Emtricitabine (Emt., a reverse transcriptase inhibitor, 100 nM) were used as controls. The mean ± SD of 2 independent experiments is shown. b Impact of Kudzu on acute replication of primary Human macrophages infected with macrophage-tropic stains ADA and 5002 M. Cells were infected with both viruses in presence of 1:100 dilution of Kudzu or 5 μg/ml of Enfurvirtide (a fusion inhibitor). Viral supernatants recovered at different times post-infection were assayed for their p24 antigen content