Fig. 3

Identification of transcription factor binding sites critical for tumorigenic HML-2 promoter activity. a Relative 5′ LTR promoter activity in the tumorigenic Hcc1954, HMLE-Ras, and HMLE-Her2 cell lines as well as the immortalized HME cell line. Promoter activity is determined as relative light units (RLU) normalized against the internal control Renilla luciferase expression and normalized against the highest expression value in the dataset. Statistical significance (dashed line, p < 0.05) was assessed by ANOVA with Bonferroni’s multiple comparisons test and is based on comparisons to HME expression. All experiments were conducted in triplicate and data displayed as the mean ± standard deviation. b The log fold change of relative transcript abundance levels, in FPKM, of transcription factors predicted to bind to unique sites on the 5′ LTR of 3q12.3 (top) and 11p15.4 (bottom). Fold change is relative to expression in the HME cell line. The log fold change of relative promoter activity (RLU) in the 5′ LTR of 3q12.3 and 11p15.4 is shown to the left of each respective plot. Highlighted in gray are the transcription factors known to bind to the HOX-PBX and RFX3 binding sites (top) as well as the transcription factors known to bind to the ATF and RORA binding sites (bottom)