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Table 2 PIC assay statistics

From: Inhibitors of the integrase–transportin-SR2 interaction block HIV nuclear import

 

Compared to DMSO

Compared to Raltegravir

n a

p valueb

 % nuclear PICsc

95% CId

n a

p valueb

% nuclear PICsc

95% CId

DMSO

 

–

–

–

49

0.8099

4.8

[3.8; 5.9]

Ral

35

0.8099 

4.5

[3.4; 5.6]

 

–

–

–

MVG010

32

0.1209

3.8

[2.3; 5.4]

29

0.1152

4.2

[2.2; 6.3]

MVG044

31

0.00001

2.1

[1.5; 2.7]

50

0.0002

2.0

[0.9; 3.2]

MVG030

30

0.0411

3.3

[2.3; 4.2]

33

0.0019

2.2

[1.4; 3.0]

MVG036

31

0.0011

2.3

[1.6; 3.1]

30

0.0700

3.2

[2.2; 4.2]

  1. Summary of two independent PIC assays comparing the four representative hit compounds to the DMSO and Ral control. HeLaP4 cells were fixed and analyzed by confocal microscopy 5 h after infection with an eGFP-labeled IN virus. PICs were assigned as cytoplasmic or nuclear based on nuclear lamina staining
  2. aNumber of HeLaP4 cells counted in each condition
  3. bp values (Mann–Whitney U test) compared to DMSO or RAL control
  4. cMean percentage of nuclear PICs in each cell calculated using a homemade MatLab routine (The MathWorks, Inc.)
  5. d95% confidence interval (CI) for the percentage of nuclear PICs
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Retrovirology

ISSN: 1742-4690

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