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Table 2 PIC assay statistics

From: Inhibitors of the integrase–transportin-SR2 interaction block HIV nuclear import

  Compared to DMSO Compared to Raltegravir
n a p valueb  % nuclear PICsc 95% CId n a p valueb % nuclear PICsc 95% CId
DMSO   49 0.8099 4.8 [3.8; 5.9]
Ral 35 0.8099  4.5 [3.4; 5.6]  
MVG010 32 0.1209 3.8 [2.3; 5.4] 29 0.1152 4.2 [2.2; 6.3]
MVG044 31 0.00001 2.1 [1.5; 2.7] 50 0.0002 2.0 [0.9; 3.2]
MVG030 30 0.0411 3.3 [2.3; 4.2] 33 0.0019 2.2 [1.4; 3.0]
MVG036 31 0.0011 2.3 [1.6; 3.1] 30 0.0700 3.2 [2.2; 4.2]
  1. Summary of two independent PIC assays comparing the four representative hit compounds to the DMSO and Ral control. HeLaP4 cells were fixed and analyzed by confocal microscopy 5 h after infection with an eGFP-labeled IN virus. PICs were assigned as cytoplasmic or nuclear based on nuclear lamina staining
  2. aNumber of HeLaP4 cells counted in each condition
  3. bp values (Mann–Whitney U test) compared to DMSO or RAL control
  4. cMean percentage of nuclear PICs in each cell calculated using a homemade MatLab routine (The MathWorks, Inc.)
  5. d95% confidence interval (CI) for the percentage of nuclear PICs