Fig. 2
From: Inhibitors of the integrase–transportin-SR2 interaction block HIV nuclear import
Cross-titration of His6-IN and GST-TRN-SR2 and evaluation of positive controls. a Both proteins were titrated against one another and the AlphaScreen signal measured (from yellow/low to red/high signal). The optimal condition containing 10 nM GST-TRN-SR2 and 40 nM His6-IN is indicated with a blue circle. GST, glutathione S-transferase; IN, integrase. b Untagged TRN-SR2 and IN were titrated out in the IN–TRN-SR2 interaction assay. Untagged IN was unable to reduce the signal to background levels most likely due to its ability to multimerize. Untagged TRN-SR2 could block the interaction completely and was selected as positive control