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Fig. 4 | Retrovirology

Fig. 4

From: Moloney leukemia virus 10 (MOV10) inhibits the degradation of APOBEC3G through interference with the Vif-mediated ubiquitin–proteasome pathway

Fig. 4

MOV10 has no influence on the binding of A3G with Vif. a, b A3G interacts with Vif or MOV10 effectively. a Human 293T cells were transfected with 2 μg of pcDNA3.1-A3G-HA (pcDNA3.1-GFP-HA as a control) and 1 μg of pcDNA3.1-Vif-FLAG and then treated with MG132 for 16 h. b Human 293T cells were transfected with 1 μg of pcDNA3.1-Vif-FLAG and 2 μg of pcDNA3.1-A3G-HA or pcDNA3.1-GFP-HA. a, b lysates from these transfected cell samples were subjected to co-immunoprecipitation analysis using anti-HA agarose beads and then detected by western blotting. c The effect of MOV10 on the interaction between A3G and Vif. 293T cells were transfected with 2 μg of pcDNA3.1-A3G-HA together with 1 μg of pcDNA3.1-Vif-FLAG, and 2 μg of pcDNA3.1-MOV10-FLAG and then treated with MG132 (4 μM) for 16 h. Samples were immunoprecipitated with anti-HA agarose beads and analyzed by western blotting. Empty vector pcDNA3.1 was used to equalize DNA amounts in each transfection. Values in c represent portions of Vif-FLAG normalized against A3G-HA relative to control values. Data in a, b, and c are representative of at least three independent experiments

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Retrovirology

ISSN: 1742-4690

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