Fig. 4
From: Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication
Codon modification but not deletion of nucleotides 22-261 or 22-378 in gag inhibits infectious virus production. a Schematic representation of p55Gag in HIV-1NL4-3, HIV-1 CM22-261, HIV-1 Δ22-261, HIV-1 CM22-378 or HIV-1 Δ22-378. b, d HeLa cells were transfected with pHIV-1NL4-3, pHIV-1 CM22-261, pHIV-1 Δ22-261, pHIV-1 CM22-378 or pHIV-1 Δ22-378 and pGFP (b) or pVSV-G (d). The amount of infectious virus in the culture supernatants was measured in TZM-bl cells. The average RLU for HIV-1NL4-3 + GFP and HIV-1NL4-3 + VSV-G is 13,701,427 and 16,981,387, respectively. The dashed line represents 1000 RLU, which is approximately the limit of the assay for reproducible differential results. c, e Gag expression in the media and cell lysate were measured by quantitative western blotting. b–e The bar charts show the average of three independent experiments relative to HIV-1NL4-3. Error bars represent standard deviation