Fig. 1
From: Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication
Codon modification of nucleotides 22-261 in gag inhibits viral replication. a Schematic representation of p55Gag in HIV-1NL4-3, HIV-1 CM22-261, HIV-1 CM22-165 and HIV-1 CM166-261. b The amount of HIV-1 CA (p24Gag) in supernatants from 293T cells transfected with pHIV-1NL4-3, pHIV-1 CM22-261, pHIV-1 CM22-165 or pHIV-1 CM166-261 were quantified by p24Gag ELISA. The bar chart is the average of three independent experiments normalized to HIV-1NL4-3. Error bars represent standard deviation. c Jurkat cells were infected with 25Â ng of p24Gag for each indicated virus. The amount of infectious virus present at each time point was measured in TZM-bl cells. This is representative of three independent experiments