Modulation of chromatin structure by the FACT histone chaperone complex regulates HIV-1 integration

Retrovirology

Table 1 Selection of cellular interact ants of the IN•viral DNA complex

Protein name	Protein family	Protein complex	Accession number	Number of peptide
LEDGF/p75	Transcription	PolII	O75475	4
PFD3 (VBP1)	Protein chaperone	Prefoldin PFD	F5H2A7	12
SMARCB1 (INI1)	Transcription	SWI/SNF	B5MC5	3
BAF	DNA binding/chromatin	–	O75531	2
FEN1	DNA repair	–	P39748	15
RAD51	DNA repair	–	Q06609	7
SSRP1	Histone chaperone	FACT	E9PPZ7	10
SPT16	Histone chaperone	FACT	Q9Y5B9	31
IWS1	Transcription	PolII	E7EX51	2
SMARCC1 (BAF155)	Transcription	SWI/SNF	Q92922	2
SMARCD1 (BAF60)	Transcription	SWI/SNF	Q96GM5	2

Cellular extracts from HeLa P4 cells were incubated with streptavidin beads coupled to fraction enriched in active IN•viral DNA complexes as shown in SI. The elution of the interacting proteins was loaded on 1256 SDS-PAGE gel stained with silver nitrate and the bands were excised from gel and submitted to electroelution. The selected proteins were identified by MS–MS. A list of proteins found only associated to IN•viral DNA complexes but not to control DNA alone and linked to chromatin or transcription is provided here. The number of peptides identified per protein is also reported. Selected proteins previously reported as playing a role in retroviral integration are underlined

ISSN: 1742-4690