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Fig. 1 | Retrovirology

Fig. 1

From: Type I interferon signaling is required for the APOBEC3/Rfv3-dependent neutralizing antibody response but not innate retrovirus restriction

Fig. 1

APOBEC3/Rfv3 inhibits infectious virus release in an IFNAR KO background. a Experimental design. B6 IFNAR KO and IFNAR/mA3 dKO mice were infected with FV and samples analyzed at the indicated time points. b Infectious viremia was measured by incubating plasma onto susceptible Mus dunni cells for 2 days and determining F-MuLV proviral DNA levels. Infectious viremia was determined for both (left) FV/LDV and (right) LDV-free FV infection. The same samples in (b) were used to determine c plasma viral RNA loads by qPCR. The ratio of the log-transformed infectious titer in (b) and plasma viral loads in (c) were used to estimate d virion infectivity. Each dot corresponds to a mouse and lines correspond to mean values. The total number of mice analyzed was combined from 2 to 3 independent experiments. Data were analyzed using a 2-tailed unpaired Student’s t test, with exact p values shown. Fold-change values in statistically-significant comparisons were based on average non-log-transformed values

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Retrovirology

ISSN: 1742-4690

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