Fig. 3

HERV-K can be effectively inhibited by Abacavir, AZT and Raltegravir: ai Hela cells and ii 293T cells were infected with HERV-K (HK) or VSV-G pseudotyped HERV-K (vsv-HK) viral particles. Total RNA was extracted 6 days post-infection and quantitative PCR was used to determine HERV-K gag mRNA expression. Glyceraldehyde 3-phosphotate dehydrogenase (GAPDH) was used as internal control and titers were expressed as fold change. iii Western blot of VSV-G protein to show that VSV-G protein is incorporated into the HERV-K viral particles. b–d Hela cells were infected with 80 pg of VSV-G pseudotyped HERV-K virus and treated with b Abacavir, c Zidovudine (AZT) or d Raltegravir, in a dose ranging from 0.05 to 0.25 µM. Six days post infection gag mRNA expression was quantified using quantitative PCR. Gag expression was compared to no treatment as control and expressed as percent inhibition. Data represent mean ± SEM of at least 3