Fig. 7
ABIN1 regulates the ubiquitination of Tat by modulating the distribution of HDM2. a HEK-293TABIN1-KO cells were transfected with plasmids encoding Myc-ABIN1 or Myc-QE2, together with Myc-HDM2, Flag-Tat or plasmid vectors as indicated, and subjected to IP as in Fig. 4a. b HeLa cells were transfected with expression vectors encoding or Myc-ABIN1, Myc-QE2, Myc-HDM2, Flag-HDM2 or Flag-Tat individually (upper panel) or in combination (middle and lower panel) as indicated for 24 h. Then cells were washed with PBS and harvested for Immunofluorescence analysis. Mouse anti-Myc and rabbit anti-Flag antibodies were used as primary antibodies, FITC-conjugated goat anti-rabbit IgG and Rhodamine-conjugated goat anti-mouse IgG were used to detect the two proteins. The consensus scale bar was 20 μm