Fig. 2

ABIN1 depletion stimulates the activity of HIV-1 promoter (Ghost-CXCR4 (X4) cells). a–e Ghost-X4 cells stably transfected a HIV-1 LTR driving GFP expression were treated with siNC, siABIN1-1, or siABIN1-2 before challenged with HIV-1(NL4-3). 48 hpi, cells were harvested and subjected to flowcytometry analysis. The effect of ABIN1 depletion on HIV-1 replication was determined by calculating the percentage of GFP positive cells, the relative mean fluorescence intensity (MFI) and relative median of GFP intensity of these GFP positive cells, over 10,000 cells were measured for each sample. a Indicates the basal level of GFP in non-infected Ghost-X4 cells. b–d Comparison of the cell counts at different GFP intensity after viral infection following ABIN1 knockdown. e The emphasis of the comparison. f, g Changing fold of MFI (f) and GFP intensity median (g) in infected cells following transfection of siRNAs targeting ABIN1 relative to siNC. The basal MFI and median of GFP intensity in siNC transfected cells were both set to 1000. h Representative of ABIN1 knockdown efficiency in Ghost-X4 cells. Data are shown as mean ± SD of triplicate samples and are representative of at least three independent experiments. p values were calculated based on unpaired t test and significant changes relative to siNC indicated. *p < 0.05; **p < 0.01; ***p < 0.001