Fig. 1

ABIN1 knockdown increases HIV-1 replication. a Jurkat T cells were transfected with siRNAs targeting ABIN1 (siABIN1-1 or siABIN1-2) or scramble siRNA (siNC) as described in “Methods”. 36 h post transfection (hpt), cells were counted and challenged with HIV-1 strain NL4-3. At 1, 3, 5 days post infection, the cells and supernatants were harvested to measure viral production by p24 ELISA of the supernatants, against a standard curve. Cells were lysed and subjected to Western Blots to determine the knockdown efficiency of ABIN1. The lower panel showed the ABIN1 knockdown efficiency at day 5 as the representative. b The experiments were conducted similarly as in (a), except that human primary CD4+ T lymphocytes were used. c, d Determination of ABIN1 mRNA level after viral infection. Jurkat cells were challenged with HIV(NL4-3) as in a, b, at 0, 24, 72 hpi, cells were harvested for RNA extraction. The mRNA levels of ABIN1 and HIV-1 gag was measured by real time PCR, normalized to cellular GAPDH. Data are shown as mean ± SD of triplicate samples and are representative of at least three independent experiments. p values were calculated based on unpaired t test and significant changes relative to siNC transfected cells or samples collected at 0 hpi. *p < 0.05; **p < 0.01; ***p < 0.001