Fig. 4
From: A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression
a env intron-containing gag and pol transcript amounts are decreased by the C+/Cn modifications. Changes in the splicing patterns of genomic and pol transcripts were analysed by RTqPCR and expressed as ratios of intron-containing transcripts divided by all env transcripts [env intron transcripts]/[env spliced + env intron]. Bars represent the mean of three independent samples and error bars the standard deviation. Locations of the primers (arrows) and probes (yellow line) are depicted above the panel. b Cotransfection of pCMVgag and -pol rescues infectivity of pHSRV13Cn. Viral titers of transfected BHK21 cells were determined on BHK21-ll cells by intracellular β-Galactosidase staining