Fig. 7

Induction of NF-κB nuclear recruitment by TLR ligands in hµglia/HIV (HC69) cells. a Representative Western blot analysis images of NF-κB p65 nuclear recruitment after stimulation. Cells [hµglia/HIV (HC69), and THP-1/HIV (HA3), as control] were untreated or treated with TNF-α (10 ng/mL), Pam3CSK4 (1 µg/mL), LPS (1 µg/mL) or poly (I:C) (1 µg/mL) for 30 min, 2, 8, or 16 h prior to nuclear extracts (NE) purification. Anti-SPT5 antibody for hµglia/HIV and anti-TBP antibody for THP-1/HIV were used as loading control. Molecular weights are indicated at the left of the blots. b Quantification of NF-κB p65 nuclear recruitment is depicted in the relative p65 band intensity (Y-axis) versus time (X-axis) graphs. TNF-α is shown in black squares, Pam3CSK4 in red circles, LPS in blue triangles, and poly (I:C) in purple triangles. Error bar represents the standard deviation of three or more experiments