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Table 1 Effect of compounds on HIV-1 gene expression and cell viability

From: Identification of small molecule modulators of HIV-1 Tat and Rev protein accumulation

  HeLa 24STNLESG CEM-GXR PBMC
  IC50 (μM) CC50
XTT (μM)
CC50
Trypan blue (μM)
IC50 CC50
XTT
IC50 CC50
FSC/SSC (μM)
CC50
ViaCount (μM)
IC50
(μM)
CC50
Trypan blue (μM)
791 8.2 >40 >40 26 μM >60 μM 4.5 μM >12 24 1.5 > 10
833 0.6 ≥40 >10 3.5 μM >20 μM 1.0 μM >5 >5 1.6 3.0
892 1.8 >40 >40 nd nd n/a 31 60 2.0 4.5
  1. The concentration of compounds that inhibit HIV gene expression by 50% (IC50) and the concentration that decreases cellular toxicity to 50% (CC50) relative to DMSO-treated cells are listed. Compound toxicity was measured by either a cellular metabolism assay (XTT). trypan blue exclusion, fluorescence-activated cell sorting (FACS) or Guava ViaCount assay. For the CEM-GXR cells, the CC50 was based on the compound toxicity tn cells infected with a Clade B HIV-1 strain, while uninfected calls were used for the ViaCount assay. FSC forward scatter, SSC side scatter, nd not done. n/a not available