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Fig. 7 | Retrovirology

Fig. 7

From: Identification of small molecule modulators of HIV-1 Tat and Rev protein accumulation

Fig. 7

791 and 833 have limited effect total protein synthesis. HeLa HIVrtTA∆Mls cells were incubated with 791, 833, or 892 for 24 h at doses indicated in the absence (−) or presence of (+) of Dox. Puromycin was added 30 min prior to harvest to measure total protein synthesis using SUnSET. a Representative blot showing the effect of the compounds on protein synthesis by puromycin labelling of nascent polypeptides (N ≥ 4). Samples not incubated with puromycin (No Puro) or treated with cycloheximide (CHX) to block translation, served as negative controls. b Quantification of protein synthesis in the presence of the compounds was measured by the volume intensity in each lane normalized to GAPDH intensity and expressed relative to the DMSO-treatment (N ≥ 4, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Error bars indicate standard error of the mean (SEM)

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