From: Ultrasensitive single-genome sequencing: accurate, targeted, next generation sequencing of HIV-1 RNA
% Majority cutoff | Method/enzyme | Total sequences | % Sequences excludeda | % Sequences remaining | % Remaining recombinants missed | % Errorb |
---|---|---|---|---|---|---|
50 | uSGS | 33,870 | 0.6 | 99.4 | 0.39 | 0.020 |
60 | 33,870 | 1.1 | 98.9 | 0.30 | 0.018 | |
70 | Kapa Hi Fi | 33,870 | 2.6 | 97.4 | 0.20 | 0.015 |
80 | Uracil+ | 33,870 | 5.8 | 94.2 | 0.13 | 0.014 |
90 | 33,870 | 28.4 | 71.6 | 0.12 | 0.010 | |
100 | 33,870 | 43.9 | 56.1 | 0.12 | 0.009 | |
50 | LP-PCR-1 | 11,008 | 4.7 | 95.3 | 4.07 | 0.033 |
60 | 11,008 | 11.7 | 88.3 | 1.86 | 0.030 | |
70 | Taq Gold | 11,008 | 27.3 | 72.7 | 0.34 | 0.020 |
80 | 11,008 | 45.0 | 55.0 | 0.20 | 0.014 | |
90 | 11,008 | 77.3 | 22.7 | <0.01 | 0.007 | |
100 | 11,008 | 87.2 | 12.8 | <0.06 | 0.006 | |
50 | LP-PCR-2 | 23,142 | 1.4 | 98.6 | 0.91 | 0.037 |
60 | 23,142 | 3.3 | 96.7 | 0.62 | 0.033 | |
70 | Kapa2G | 23,142 | 10.5 | 89.5 | 0.30 | 0.024 |
80 | Robust | 23,142 | 25.4 | 74.6 | 0.14 | 0.020 |
90 | 23,142 | 63.5 | 36.5 | 0.09 | 0.013 | |
100 | 23,142 | 85.7 | 14.3 | 0.15 | 0.009 | |
50 | LP-PCR-3 | 20,252 | 4.6 | 95.4 | 6.44 | 0.016 |
60 | 20,252 | 13.8 | 86.2 | 2.00 | 0.015 | |
70 | Platinum | 20,252 | 23.4 | 76.6 | 0.35 | 0.013 |
80 | Hi Fi Taq | 20,252 | 34.8 | 65.3 | 0.15 | 0.011 |
90 | 20,252 | 71.7 | 28.3 | <0.04 | 0.004 | |
100 | 20,252 | 92.0 | 8.0 | <0.07 | 0.004 |